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coordinated enzymes中文是什么意思

  • 协调酶

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  • When both genes were co - expressed in e . coli , the activity of ppsa varied from 2 . 1 - 9 . 1 fold comparing to control , but the activity of tkta was relatively stable ( 3 . 9 - 4 . 5 fold ) . whatever the two genes were expressed respectively or cooperatively , both could promote the production of dahp , the first intermediate of the common aromatic pathway , but co - expression was more effective on forming dahp and screened ppt - and ptp - as more effective . the results demonstrate that co - expression of ppsa and tkta can improve the production of dahp , and what ' s more , when multigenes co - expressed , the recombinant which has coordinated enzymes activity is optimum
    莽草酸途径的最优化和整体调控基因csra的敲除正是上述改变的分子基础,同时也为三种芳香族氨基酸的基因工程菌的构建打下了基础; 7 .在国内外首次实现了共同途径限制性底物关键酶ppsa刁无‘及arog与分支途径关键酶基因phea的串联高效表达,所构建的重组质粒ptga ,其ppsa 、 tkta 、 arog 、 cm和pd的酶活分别比对照提高了3 、 2 、 2 , 5 、 4 、 2 . 3倍,且其酶活比较协调一致; 8 .将ptga导入到筛选的基因敲除和基因替换菌株大肠杆菌31884 c甲b中,摇瓶发酵证实比以往所构建的基因工程菌株具有较高的phe产量和糖转化率率,分别为0 . 448 %和22 . 4 % 。
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Last modified time:Fri, 15 Aug 2025 00:29:56 GMT

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